Testing antimicrobial susceptibilities of adherent bacteria by a method that incorporates guidelines of the National Committee for Clinical Laboratory Standards.

نویسندگان

  • G Domingue
  • B Ellis
  • M Dasgupta
  • J W Costerton
چکیده

Membrane filters (0.22-microns pore size) were colonized with 10(4) CFU of logarithmic-phase bacteria per filter under laminar flow conditions in a Modified Robbins Device. Colonized filters were then placed upon agar dilution plates for MIC determinations. Subsequently, filters were transferred to control plates and incubated to obtain MBCs.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

In vitro susceptibility tests for cationic peptides: comparison of broth microdilution methods for bacteria that grow aerobically.

The in vitro susceptibilities of 90 clinical isolates of gram-positive and gram-negative aerobic bacteria to six cationic peptides, buforin II, cecropin P1, indolicidin, magainin II, nisin, and ranalexin, were evaluated by two broth microdilution methods. The first method was performed according to the procedures outlined by the National Committee for Clinical Laboratory Standards for bacteria ...

متن کامل

Frequency of Pathogens and Antimicrobial Susceptibility of Bacteria Isolated from Bloodstream Infections

  Background and Objectives: Blood stream infections cause significant morbidity and mortality in the world. In this study, we aimed at describing the organisms responsible for septicemia in 2006-2007 and their antimicrobial susceptibility that might lead to proper selection of antimicrobial agents in hospitalized patients with suspected sepsis. Materials and Methods: Microbiology reports of ...

متن کامل

مقایسه روش تعیین حساسیت دیسک دیفیوژن و واکنش زنجیره ای پلیمراز برای شناسایی استافیلوکوکوس مقاوم به متی سیلین

Background: Staphylococci as a micro-organism, has the most importance to cause nosocomial infections, particularly in patients with indwelling catheters or other medical devices. Unfortunately 90% of Staphylococci isolated from the nosocomial infections are resistant to methicillin, and methicillin resistance strains are also resistant to a wide range of antimicrobial drugs, therefore detectin...

متن کامل

Comparison of Disk Diffusion and E-Test with the Reference Method of Microbroth Dilution for Susceptibility Testing of Acinetobacter baumannii Isolates to Tetracyclines

Abstract       Background and Objective: Due to the continuous increase of multidrug-resistant Acinetobacter baumannii strains around the world, decision making for an effective treatment of infections caused by this organism depends on the results of antimicrobial susceptibility tests. In this study, the validity of disk diffusion and E-test methods was assessed b...

متن کامل

تعیین فراوانی و میزان حساسیت آنتی‌بیوتیکی باکتری‌های جدا شده از کشت‌های ادرار، مدفوع و خون ارسالی به آزمایشگاه‌های دانشگاه علوم پزشکی رفسنجان در سال1383

Background and Objectives: Antimicrobial susceptibility pattern of bacterial agents isolated from clinical samples in different parts of the country provides essential information regarding the selection of antibiotics for all patients living in different areas. Unfortunately data about the frequency and also antimicrobial resistance pattern of phathogen agents in clinical samples is rare in ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of clinical microbiology

دوره 32 10  شماره 

صفحات  -

تاریخ انتشار 1994